Cell cycle‐dependent association of Gag‐Mil and hsp90

FEBS Letters

Lovrić, Josip¹  Bischof, Oliver²  Moelling, Karin¹ 

[1] Max-Planck-Institut für Molekulare Genetik, Abt. Schuster, Ihnestrasse 73, D-14195 Berlin (Dahlem, Germany;Max-Delbrück-Centrum für Molekulare Medizin, Robert-Rössle-Strasse 10, D-13125 Berlin, Germany

关键词: hsp90;    Gag-Mil;    c-Raf-1;    Cell cycle;    Microsequencing;   

DOI  :  10.1016/0014-5793(94)80598-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Immunoprecipitated p100Gag-Mil protein kinase from MH2-transformed quail embryo fibroblasts is associated with an 89 kDa protein. The molar ratio between p89 and Gag-Mil in the immunocomplex is 0.72, indicating that the majority of Gag-Mil is complexed with p89. During mitosis part of Gag-Mil is shifted to a form with reduced electrophoretic mobility, p102Gag-Mil. Appearance of p102Gag-Mil leads to a reduced association with p89 indicating that p102 is not associated with p89. Microsequencing of p89 isolated from immunoprecipitates of Gag-Mil identified the protein as the quail homologue of chicken hsp90. Our results show that p100Gag-Mil is associated with hsp90 with a high stoichiometry and that upshifted p102Gag-Mil is released from the complex with hsp90.

【 授权许可】

Unknown