The intrinsic 22 kDa protein is a chlorophyll‐binding subunit of photosystem II

FEBS Letters

Funk, Christiane²  Andersson, Bertil²  Renger, Gernot¹  Schröder, Wolfgang P.²  Green, Beverley R.² 

[1] Max-Volmer Institute, Technical University Berlin, Strasse des 17 Juni 135, D-10623 Berlin, Germany;Department of Biochemistry, Arrhenius Laboratories for Natural Sciences, Stockholm University, S-106 91 Stockholm, Sweden

关键词: Chlorophyll-protein;    psbS gene product;    22 kDa protein;    Photosystem II;    Spinach;    BBY;    photosystem II membrane fragments;    CAB;    chlorophyll a/b binding;    Chl;    chlorophyll;    CP;    chlorophyll-binding protein;    ELIP;    early light induced protein;    MES;    4-morpholine-ethanesulphonic acid;    OGP;    octyl-glucopyranoside;    OTG;    octyl-thio-glucopyranoside;    PSII;    photosystem II;    PVDF;    polyvinylidenefluoride;    SDS-PAGE: SDS-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(94)80513-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The intrinsic 22 kDa polypeptide associated with photosystem II (psbS protein) was found to be able to bind chlorophyll. Extraction of isolated photosystem II membranes with octyl-thioglucopyranoside, followed by repetitive electrophoresis under partially denaturing conditions gave only one green band. It contained both chlorophyll a and chlorophyll b, exhibited an absorption maximum at 674 nm and a 77 K fluorescence peak at 675 nm. The chlorophyll-protein band contained a single polypeptide of 22 kDa. Based on these results and on previous protein sequence comparisons, it is suggested that the psbS protein is a chlorophyll a/b binding polypeptide and should thus be denoted CP22.

【 授权许可】

Unknown