The minor antenna complexes of an oxygen evolving photosystem II preparation: purification and stoichiometry

FEBS Letters

Giardi, Maria Teresa²  Barbato, Roberto¹  Giacometti, Giorgio Mario¹  Rigoni, Fernanda¹ 

[1] Dipartimento di Biologia, Università di Padova, Via Trieste 75, 35121 Padova Italy;IREV-CNR, Via Salaria km 29.3, 00016 Monterotondo Scalo Roma, Italy

关键词: Photosystem II;    Chlorophyll-protein;    Minor antenna;    Stoichiometry;    (Spinach);    DCMU;    3-(3;    4-dichlorophenyl)-1;    1-dimethylurea;    Ioxynil;    3;    5-diiodo-4-hydroxybenzonitrile;    OEE;    oxygen evolving enhancer;    PAGE;    polyacrylamide gel electrophoresis;    PS;    photosystem;    Tricine;    N-(tris[hydroxymethyl]-methyl)-glycine;   

DOI  :  10.1016/0014-5793(89)81445-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A photosystem II complex obtained by partial solubilization of spinach PS II membranes has been analyzed in terms of chlorophyll-protein complexes. The complex was found to contain the minor antennas CP29 and CP26 and a very small amount of LHC II (less than 3%). No trace of CP24 was found to be associated with the PS II complex. This chlorophyll a/b-protein was instead found in the fraction containing the main light-harvesting complex LHC II. The stoichiometry of the minor antennas indicates the values 3:3:1 for the ratios CP29:CP26:P680.

【 授权许可】

Unknown