Cardiac L‐type Ca2+ channel triggers transmitter release in PC 12 cells

FEBS Letters

De Jongh, Karen S.¹  Atlas, Daphne²  Schwartz, Lydia²  Avidor, Boaz²  Avidor, Tomer² 

[1] University of Washington, Seattle, USA;Department of Biological Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel

关键词: cDNA cloning;    Dihydropyridine receptor;    N-type Ca2+ channel;    L-type Ca2+ channel;    PC12 cell;    DHP;    dihydropyridine;    ω-CgTx;    ω-conotoxin GVIA;    ω-AgaTx;    ω Agatoxin IVA;    [3H]DA;    [3H]dopamine;   

DOI  :  10.1016/0014-5793(94)80502-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Among the various voltage-sensitive Ca²⁺ channels present in PC 12 cells are the dihydropyridine (DHP)-sensitive L-channel, the ω-conotoxin (ω-CgTx)-sensitive N-channel, and an atypical ω-CgTx/DHP-insensitive Ca²⁺ channel. Depolarization-evoked Ca²⁺ entry and [³H]dopamine release is mediated by L-type Ca²⁺ channels determined by the use of Ca²⁺ channel antagonists, and a single protein of 250 kDa is recognized by L-type-specific antibodies. Screening of a PC12 cDNA library revealed two types of Ca²⁺channels which were identified by partial sequencing. A pc12-L clone displayed virtually identical sequence homology to the cardiac L-type channel. The identical sequence homology of the single alternative splicing region confirmed clone pc12-L as the rbC-I transcript, a cardiac-neuronal α1 subunit expressed in rat brain. Clone pc12-N displayed identical sequence homology to rbB-I, a neuronal al subunit of the N-type Ca²⁺ channel expressed in rat brain; Northern blot analysis identified RNA of a size similar to that previously described for rat brain.

【 授权许可】

Unknown