Biochemical, pharmacological and genomic characterisation of Ts IV, an α‐toxin from the venom of the South American scorpion Tityus serrulatus

FEBS Letters

Bougis, P.E.²  Ribeiro, A.M.¹  Rochat, H.²  Diniz, C.R.³  Martin-Eauclaire, M.F.²  Céard, B.² 

[1] Departamento de Bioquimica e Immunologia, Institute de Sciencias Biologicas, Universidade Federal de Minais Gerais, Campus Pampula, 30000 Belo Horizonte MG, Brazil;Laboratoire de Biochimie, CNRS URA 1455, Faculté de Médecine secteur Nord, Boulevard Pierre Dramard, 13916 Marseille Cédex 20, France;Fundaçao Ezequiel Diaz, Rua Conde Pereira Carneiro 80, 30550 Belo Horizonte MG, Brazil

关键词: α- and β-Scorpion toxin;    cDNA cloning;    Tityus serrulatus;   

DOI  :  10.1016/0014-5793(94)80496-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The venom of the scorpion, Tityus serrulatus, was fractionated to investigate the chemical and pharmacological properties of its α-toxin content. Three α-toxins (Ts III, Ts IV and Ts V) were purified by conventional chromatography (gel filtration and ion-exchange chromatography), followed by immunoaffinity chromatography. Competition experiments using reference α- and β-toxins suggested that these α-toxins were contaminated with around 0.01% of β-toxin. The sequence of the first 30 amino acids of Ts IV was established. Using an oligonucleotide probe, a cDNA encoding its precursor was cloned from a venom gland cDNA library. The primary structure deduced from the cDNA nucleotide sequence provides possible explanations for the polymorphism of these three molecules.

【 授权许可】

Unknown