Primary structure of toxin IV of Leiurus quinquestriatus quinquestriatus

FEBS Letters

Kopeyan, Charles¹  Rochat, Hervé¹  Martinez, Gérard¹ 

[1] INSERM U. 172 et CNRS U A 553, Laboratoire de Biochimie, Faculté de Médecine, Secteur Nord, Boulevard Pierre-Dramard, 13326 Marseille Cédex 15, France

关键词: Scorpion toxin;    Amino acid sequence;    Automatic sequencing;    Scorpion toxin classification;    AaH;    Androctonus australis Hector;    Bot;    Buthus occitanus tunetanus;    Amm;    Androctonus mauretanicus mauretanicus;    Lqq;    Leiurus quinquestriatus quinquestriatus;    Be;    Buthus epeus;    CsE;    Centruroides sculpturatus Ewing;    Css;    Centruroides suffusus suffusus;    Ts;    Tityus serrulatus;    AaH I;    AaH I' (28) - AaH I' ' (29) - AaH II (30) - AaH III (19) - Bot I and Bot II (25) - Bot III (31) - Lqq V (11) - Amm V (32) - Be M10 (33) - CsE I (34) - Css II (5) - Ts VII (22);   

DOI  :  10.1016/0014-5793(85)80262-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report the sequence of toxin IV of Leiurus quinquestriatus quinquestriatus, one of the five toxins lethal for the mouse purified from the venom of this scorpion. Automatic sequencing of the S-carboxymethylated protein and derived peptides obtained by action of the Staphylococcus aureus protease allowed us to work out the complete sequence of 65 residues. The toxin which was found to be blocked at the C-terminal end has an extra residue at the N-terminus as compared with other toxins from Asian and African scorpions. Moreover, toxin IV must be classified as an α-scorpion toxin since it was shown to displace competitively ¹²⁵I-toxin II of Androctonus australis Hector (¹²⁵I-AaH II) from its binding site on rat brain synaptosomes. Nevertheless, it cannot be classified in one of the groups of scorpion toxins previously defined on the level of their antigenic properties. Again the classifications of scorpion toxins based on sequence homologies or antigenic properties support one another.

【 授权许可】

Unknown