A large number of novel Ergtoxin‐like genes and ERG K+‐channels blocking peptides from scorpions of the genus Centruroides

FEBS Letters

Wanke, Enzo¹  Cassulini, Rita Restano¹  Coronas, Fredy I.V.¹  Corona, Miguel¹  Possani, Lourival D.¹  Valdez-Cruz, Norma A.¹  Zamudio, Fernando Z.¹  Merino, Enrique¹  Ramı́rez-Domı́nguez, Martha E.¹  Garcı́a, Blanca¹  Gurrola, Georgina B.¹ 

[1] Institute of Biotechnology, National Autonomous University of Mexico, Avenida Universidad, 2001, P.O. Box 510-3, Cuernavaca 62210, Mexico

关键词: Centruroides;    ERG-channel;    Ergtoxin;    K+-channel;    Nomenclature;    Phylogeny;    Scorpion toxin;   

DOI  :  10.1016/S0014-5793(02)03652-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Twenty-three novel sequences similar to Ergtoxin (ErgTx) were obtained by direct sequencing of peptides or deduced from gene cloned using cDNAs of venomous glands of Centruroides (C.) elegans, C. exilicauda, C. gracilis, C. limpidus limpidus, C. noxius and C. sculpturatus. These peptides have from 42 to 47 amino acid residues cross-linked by four disulfide bridges. They share sequence similarities (60–98% compared with ErgTx1) and were shown to block ERG K⁺-channels of F-11 clone (N18TG-2×rat DRG) cultured cells. An unrooted phylogenetic tree analysis of these peptides showed that they conform at least five different subfamilies, of which three are novel subfamilies.

【 授权许可】

Unknown