Oxidation of LDL by nitric oxide and its modification by Superoxides in macrophage and cell‐free systems

FEBS Letters

Chow, Song-Nan²  Wang, Jin-Mei¹  Lin, Jen-Kun¹ 

[1] Institutes of Biochemistry, College of Medicine, National Taiwan University, No. 1, Section 1, Jen-ai Road, Taipei, Taiwan, Republic of China;Department of Obstetrics and Gynecology, College of Medicine, National Taiwan University, No. 1, Section 1, Jen-ai Road, Taipei, Taiwan, Republic of China

关键词: Nitric oxide;    Superoxide;    Macrophage;    Lipoprotein;    Lipid peroxidation;   

DOI  :  10.1016/0014-5793(94)80494-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Oxidative modification of low density lipoproteins (LDL) can increase the atherogenicity of LDL. Here we demonstrate the potential role of nitric oxide (NO) in LDL modification. Dose-dependent alterations of LDL including an increase in electrophoretic mobility, generation of peroxides and degradation of apoprotein B occurred during incubation with NO. Sodium nitroprusside was also shown to increase the electromobility of LDL. Superoxide dismutase (SOD) and catalase did not affect this result. NO treatment also increases the negative charges on other lipoproteins. In addition, we have demonstrated that NO plays a protective role in macrophage or superoxide-induced LDL modification through its neutralizing action.

【 授权许可】

Unknown