Peroxynitrite releases copper from caeruloplasmin: implications for atherosclerosis

FEBS Letters

Darley-Usmar, Victor²  Swain, Jason A.¹  Gutteridge, John M.C.¹ 

[1] Unit of Critical Care, Department of Anaesthesia and Intensive Care, Royal Brompton Hospital National Heart & Lung Institute, Sydney Street, London, SW3 6NP, UK;Department of Biochemical Sciences, Wellcome Research Laboratories, Beckenham, Kent, BR3 3BS, UK

关键词: Peroxynitrite;    Caeruloplasmin;    Reactive plasma copper;    Atherosclerosis;    Ferroxidase activity;   

DOI  :  10.1016/0014-5793(94)80582-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Peroxynitrite may be formed in the vasculature by the reaction of puperoxide with nitric oxide. When the blue copper-containing protein, caeruloplasmin, is incubated with peroxynitrite, copper is released, and ferroxidase activity and the blue colouration are lost. When plasma from normal subjects is incubated with peroxynitrite, the oxidant reacts with numerous plasma constituents but is still able to release copper from caeruloplasmin. As the ferroxidase activity of caeruloplasmin is lost in plasma in the presence of peroxynitrite, a second ferroxidase activity associated with peroxidised lipids, and not inhibited by azide, is formed.

【 授权许可】

Unknown