Modification of tissue factor by peroxynitrite influences its procoagulant activity

FEBS Letters

Bruckdorfer, K.R.¹  Ettelaie, C.¹  Bradley, N.J.¹  Naseem, K.M.¹  Adam, J.M.¹  James, N.J.¹ 

[1] Department of Biochemistry and Molecular Biology, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PF, UK

关键词: Atherosclerosis;    Peroxynitrite;    Tissue factor;    Procoagulant activity;    Nitration;   

DOI  :  10.1016/S0014-5793(98)00627-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Peroxynitrite, a reactive oxidising species resulting from a reaction between nitric oxide and the superoxide anion, modifies proteins by nitration of certain amino acids such as tyrosine. Tissue factor (TF), a transmembrane protein, is expressed on cells under inflammatory conditions and initiates the coagulation cascade. The extracellular domain of TF is rich in tyrosine. Exposure of recombinant TF and cellular TF to peroxynitrite was associated with a reduction in procoagulant activity. This was accompanied by an elevated level of nitrotyrosine residues. Peroxynitrite may have a protective role by attenuation of the thrombotic properties of TF.

【 授权许可】

Unknown