FEBS Letters | |
Nitric oxide blocks the cell cycle of mouse macrophage‐like cells in the early G2+M phase | |
Takagi, Kuniaki3  Suketa, Yasunobu2  Isobe, Yuji3  Okouchi, Etsuko2  Yasukawa, Kiyoshi1  | |
[1] Biotechnology Research Laboratory, Toso Corporation, 2743-1, Hayakawa, Ayase, Kanagawa 252, Japan;Laboratory for Cell Biology, Pharma Research Laboratories, Hoechst Japan Limited, 1-3-2, Minamidai, Kawagoe, Saitama 350, Japan;Department of Environmental Biochemistry, School of Pharmaceutical Science, University of Shizuoka, Yada 52-1, Shizuoka, 422, Japan | |
关键词: Cell cycle; Nitric oxide; Interleukin-6; Macrophage like cell; Microfilament; | |
DOI : 10.1016/0014-5793(94)80128-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The effects of nitric oxide produced by macrophage-like cells (Mml) on the cell cycle were investigated. Mml cells lost proliferative activity in the presence of interleukin-6 (IL-6) and a subpopulation accumulated in the G2+ M phase. This level increased in proportion to the incubation time. The DNA content of the cells was slightly lower than that of Mml cells treated with vinbrastine or demecolcine, drugs which block the cell cycle in the M phase. The peak of the early G2+M phase was reduced by treatment with N G-mono-methyl-l-arginine. However, after treatment with exogenous nitric oxide or sodium nitroprusside, the G0/G1 phase increased, but the early-G2+M and the S phase decreased. The flow cytometry pattern in IL-6-treated Mml was the same as that of cytochalasin B-treated Mml. These data suggest that endogenous nitric oxide affects the microfilament system of IL-6-treated Mml cells and blocks the cell cycle in the early G2+M phase.
【 授权许可】
Unknown
【 预 览 】
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