Insulin‐like growth factors counteract the effect of interleukin 1β on type II phospholipase A2 expression and arachidonic acid release by rabbit articular chondrocytes

FEBS Letters

Thomas, G.²  Poiraudeau, S.¹  Masliah, J.²  Béréziat, G.²  Berenbaum, F.²  Corvol, M.T.¹ 

[1] Unité INSERM 30, Tour Lavoisier, Hôpital des Enfants Malades, 149 rue de Sèvres, 75743 Paris cédex 15, France;URA CNRS 1283, Faculté de Médecine Saint Antoine, 27 rue Chaligny, 75012 Paris, France

关键词: Phospholipase A2;    Arachidonic acid;    Chondrocyte;    Interleukin 1β;    Insulin-like growth factor;    Arthritis;    AA: arachidonic acid;    IGF: insulin-like growth factor;    IL1β: interleukin 1β;    PLA2: phospholipase A2;    sPLA2: secreted PLA2;    cPLA2: cytosolic PLA2;   

DOI  :  10.1016/0014-5793(94)80171-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Interleukin 1β was found to stimulate arachidonic acid release, and the synthesis and secretion of type II phospholipase A2 by rabbit articular chondrocytes in vitro. Interleukin 1β had no effect on the level of cytosolic phospholipase A2 mRNA. Insulin-like growth factors, which help stabilize the cartilage matrix, reduced the effect of interleukin 1β on type II phospholipase A2 activity and mRNA level, and decreased the Interleukin 1β-stimulated arachidonic acid release to the basal values. This suggests that type II phospholipase A2 plays a key role in arachidonic acid release from rabbit articular chondrocytes and that insulin-like growth factors counteract the effect of interleukin 1β. They may therefore be considered as potential antiinflammatory agents.

【 授权许可】

Unknown