Polyunsaturated fatty acids potentiate interleukin‐1‐stimulated arachidonic acid release by cells overexpressing type IIA secretory phospholipase A2

FEBS Letters

Kambe, Terumi¹  Murakami, Makoto¹  Kudo, Ichiro¹ 

[1] Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan

关键词: Phospholipase A2;    Arachidonic acid;    Prostaglandin;    PLA2;    phospholipase A2;    sPLA2-IIA;    type IIA secretory PLA2;    cPLA2;    cytosolic PLA2;    iPLA2;    Ca2+-independent PLA2;    COX;    cyclooxygenase;    AA;    arachidonic acid;    PUFA;    polyunsaturated fatty acid;    PG;    prostaglandin;    IL-1;    interleukin-1;    FCS;    fetal calf serum;    HETE;    hydroxyeicosatetraenoic acid;    MAFP;    methyl arachidonylfluorophosphate;   

DOI  :  10.1016/S0014-5793(99)00702-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

By analyzing human embryonic kidney 293 cell transfectants stably overexpressing various types of phospholipase A₂ (PLA₂), we have shown that polyunsaturated fatty acids (PUFAs) preferentially activate type IIA secretory PLA₂ (sPLA₂-IIA)-mediated arachidonic acid (AA) release from interleukin-1 (IL-1)-stimulated cells. When 293 cells prelabeled with [³H]AA were incubated with exogenous PUFAs in the presence of IL-1 and serum, there was a significant increase in [³H]AA release (in the order AA>linoleic acid>oleic acid), which was augmented markedly by sPLA₂-IIA and modestly by type IV cytosolic PLA₂ (cPLA₂), but only minimally by type VI Ca²⁺-independent PLA₂, overexpression. Transfection of cPLA₂ into sPLA₂-IIA-expressing cells produced a synergistic increase in IL-1-dependent [³H]AA release and subsequent prostaglandin production. Our results support the proposal that prior production of AA by cPLA₂ in cytokine-stimulated cells destabilizes the cellular membranes, thereby rendering them more susceptible to subsequent hydrolysis by sPLA₂-IIA.

【 授权许可】

Unknown