Glucose transporter localization in rat skeletal muscle

FEBS Letters

Wilson, Cindy M.²  Cushman, S.W.²  Dohm, G.Lynis³  Dudek, Ronald W.⁴  Holman, Geoffrey D.¹ 

[1] Department of Biochemsitry, University of Bath, Bath, UK;Experimental Diabetes, Metabolism and Nutrition Section, DB/NIDDK National Institute of Health, Bethesda, MD, USA;Department of Biochemistry, School of Medicine, East Carolina University, Greenville, NC 27858, USA;Department of Cell Biology, School of Medicine, East Carolina University, Greenville, NC 27858, USA

关键词: GLUT4;    Glucose transport;    Skeletal muscle;    Bis-mannose;    Photoaffinity label;   

DOI  :  10.1016/0014-5793(94)80416-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Surface glucose transporters of intact muscles were photolabeled with the membrane impenneant ATB-[2-³H]BMPA reagent and localized by autoradiography. We found sparse labeling of the glucose transporters by ATB-[2-³H]BMPA on the sarcolemmal membrane around the muscle fiber. The majority of label was on the interior of the muscle fiber, at a discrete site which matched the distribution of AI junctions and which was presumed to be on the exterior surface of T-tubules. The amount of photolabel on the T-tubule was increased in response to insulin and wasblocked by cytochalasin B. These results support the concept that glucose transport may occur predominately across the T-tubule membrane under basal and insulin-stimulated conditions.

【 授权许可】

Unknown