Regulation of cell surface GLUT1, GLUT3, and GLUT4 by insulin and IGF‐I in L6 myotubes

FEBS Letters

Wilson, Cindy M.²  Mitsumoto, Yasuhide¹  Klip, Amira¹  Maher, Fran² 

[1] Division of Cell Biology, The Hospital for Sick Children, 555 University Ave., Toronto, Ont. M5G 1X8, Canada;Experimental Diabetes, Metabolism and Nutrition Section, DB, NIDDK National Institutes of Health, Bldg. 10, Rm. 5N102, 10 Center DR MSC 1420, Bethesda, MD 20892-1420, USA

关键词: L6 myotube;    GLUT1;    GLUT3;    GLUT4;    Glucose transport;    Bis-mannose photolabel;   

DOI  :  10.1016/0014-5793(95)00589-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effects of insulin and IGF-I on the cell surface quantities of GLUT1, GLUT3 and GLUT4 glucose transporters in L6 myotubes were determined with the exofacial bis-mannose phololabel (ATB-BMPA). In basal cells, an equal molar quantity of each transporter isoform was found at the cell surface. Insulin stimulated the translocation of all three glucose transporter isoforms to the plasma membrane fraction from the light microsome fraction, resulting in equal molar quantities on the cell surface. IGF-I stimulated a similar translocation of all isoforms, augmented by an increase in surface GLUT3 as assessed by ATB-BMPA.

【 授权许可】

Unknown