Identification of a cDNA encoding a Drosophila calcium/calmodulin regulated protein phosphatase, which has its most abundant expression in the early embryo

FEBS Letters

Chen, Mao Xiang¹  Cohen, Patricia T.W.¹  Brown, Linda¹ 

[1] Medical Research Council Phosphorylation Unit, Department of Biochemistry, The University, Dundee DD1 4HN, Scotland, UK

关键词: Protein phosphatase 2B;    Calcineurin;    cDNA sequence;    Calcium;    Calmodulin;    Drosophila melanogaster;    PP;    protein phosphatase;    PCR;    polymerase chain reaction;   

DOI  :  10.1016/0014-5793(94)80398-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A 3.3 kb cDNA encoding the complete amino acid sequence of a calcium/calmodulin regulated protein phosphatase has been isolated from a Drosophila eye disc cDNA library. The predicted protein of 560 amino acids (molecular mass 62 kDa) is 73–78% identical to human PP2B isoforms. The cDNA hybridised to the X-chromosome at cytological position 14D1-4. Two transcripts of 3.5 kb and 3.0 kb were expressed during embryonic development, their levels being highest in the early embryo. The larger transcript was also clearly present in adult females. This pattern of expression indicates a role for calcium/calmodulin regulated protein phosphatase in embryonic development.

【 授权许可】

Unknown