FEBS Letters | |
Structure and stability of pertussis toxin studied by in situ atomic force microscopy | |
Shao, Zhifeng1  Mou, Jianxun1  Yang, Jie1  | |
[1]Bio-SPM Laboratory, Department of Molecular Physiology & Biological Physics and Biophysics Program, University of Virginia, Box 449, Charlottesville, VA 22908, USA | |
关键词: Atomic force microscopy; Pertussis toxin; Structure; Resolution; | |
DOI : 10.1016/0014-5793(94)80122-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Pertussis toxin, both complete and the B-oligomer, were imaged by atomic force miroscopy (AFM), using specimens prepared by simple surface adsorption on mica without further manipulation. The spatial arrangement of the subunits of the B-oligomer was clearly resolved, representing the first protein quaternary structure obtained by AFM in situ. The results suggest that the B-oligomer is a flat pentamer with the two large subunits located next to each other, and the catalytic A-subunit situated at the center above. We found that the B-pentamer was structurally stable for temperatures up to 60°C and within the pH range of 4.5–9.5. It is also demonstrated that the AFM was capable of resolving features down to 0.5 nm on the B-oligomers, indicating its great potential for structural determination.
【 授权许可】
Unknown
【 预 览 】
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RO201912020299085ZK.pdf | 1399KB | download |