Nitric oxide donor GEA 3162 inhibits endothelial cell‐mediated oxidation of low density lipoprotein

FEBS Letters

Vuorinen, Pauli¹  Ylä-Herttuala, Seppo¹  Metsä-Ketelä, Timo¹  Nikkari, Tapio¹  Jaakkola, Olli¹  Malo-Ranta, Ulla¹  Moilanen, Eeva¹ 

[1] Department of Biomedical Sciences, University of Tampere, P.O. Box 607, 33101 Tampere, Finland

关键词: Atherosclerosis;    Endothelial cell;    Nitric oxide;    Oxidized low density lipoprotein;    SIN-1;    EC;    endothelial cells;    DMEM;    Dulbecco's modified Eagle's medium;    LDL;    low density lipoprotein;    LPDS;    lipoprotein-deficient serum;    MDA;    malondialdehyde;    NO;    nitric oxide;    O2 −;    superoxide anion;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;    TBARS;    thiobarbituric acid-reactive substances;    SIN-1;    3-morpholinosydnonimine-hydrochloride;   

DOI  :  10.1016/0014-5793(94)80269-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of a nitric oxide (NO) donor GEA 3162 on the endothelial cell (EC)-mediated oxidation of low density lipoprotein (LDL) was studied. In comparison to LDL incubated with EC without GEA 3162, the presence of GEA 3162 inhibited LDL oxidation by EC, as indicated by the following findings. (a) The degradation rate of LDL in macrophages was reduced to control levels. (b) The electrophoretic mobility of LDL decreased in a dose-dependent manner, (c) The concentrations of thiobarbituric acid-reactive substances and hydroperoxide-derived hydroxy fatty acids were lower. (d) The breakdown of apolipoprotein B was reduced. The results indicate that GEA 3162 prevents EC-mediated oxidation of LDL.

【 授权许可】

Unknown