Noncanonical Oct‐sequences are targets for mouse Oct‐2B transcription factor

FEBS Letters

Stepchenko, A.G.¹ 

[1] Engelhardt Institute of Molecular Biology, The Russian Academy of Sciences, Vavilov sir. 32, Moscow, 117984, Russian Federation

关键词: Mouse Oct-2B;    DNA-protein interaction;    POU domain;    Octamer sequence;   

DOI  :  10.1016/0014-5793(94)80268-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have suggested a random modification method for determining preferable binding sites of a DNA-binding protein and applied this method to the Oct-2B transcription factor. Our results indicate that the Oct-2B protein interacts with canonical oct sequence ATGC/TAAAT and degenerated sequences which contain TAAT motif in the binding site. We have determined nucleotides in the binding sites, involved in the DNA-protein interaction, and the equilibrium dissociation constants K _d for these sequences. These data show that a much greater number of potential targets for Oct proteins exist on DNA and changed our view on the gene expression regulation by this protein factor.

【 授权许可】

Unknown