Secondary structure of the oct‐3 POU homeodomain as determined by 1H‐15N NMR spectroscopy

FEBS Letters

Hayashi, Fumiaki¹  Shirakawa, Masahiro²  Morita, Eugene H.²  Imagawa, Masayoshi³  Kyogoku, Yoshimasa² 

[1]Shionogi Research Laboratories, Shionogi & Co., Fukushima-ku, Osaka 553, Japan

[2]Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565, Japan

[3]School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565, Japan

关键词: POU domain;    3D NMR;    Homeodomain;    Secondary structure;    Oct-3;   

DOI  :  10.1016/0014-5793(93)80088-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Most of the ¹H and ¹⁵N magnetic resonances of the 66 amino acid long POU homeodomain of mouse Oct-3 have been assigned by the combined use of the two-dimensional homonuclear, and two- and three-dimensional heteronuclear NMR methods. The sequential NOE connectivities and amide proton exchange measurements indicate the presence of three helical regions within the domain. The positions of the three helices correspond well to those of other homeodomains, the three-dimensional structures of which have already been determined. The present NMR study provides the first experimental evidence for the existence of a helix-turn-helix motif in the oct-3 POU homeodomain.

【 授权许可】

Unknown