POU‐specific domain of Oct‐2 factor confers ‘octamer’ motif DNA binding specificity on heterologous Antennapedia homeodomain

FEBS Letters

Schaffner, Walter¹  Xu, Licen¹  Brugnera, Enrico¹  Arnosti, David N.¹ 

[1] Institute of Molecular Biology II, University of Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland

关键词: POU domain;    DNA binding protein;    Homeodomain;    Octamer factor;   

DOI  :  10.1016/0014-5793(92)81506-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The bipartite DNA binding domain of the POU family of transcription factors contains a ‘POU-specific’ domain unique to this class of factors and a ‘POU homeodomain’ homologous to other homeodomains. We compared DNA binding of the Oct-2 factor POU domain and the Antennapedia (Antp) homeodomain with a chimeric Oct-2/Antp protein in which the distantly related Antp homeodomain was substituted for the Oct-2 POU homeodomain. The Oct-2/Antp chimeric protein bound both the octamer and the Antp sites efficiently, indicating that DNA binding specificity is contributed by both components of the POU domain.

【 授权许可】

Unknown