The human sperm protein PH‐20 has hyaluronidase activity

FEBS Letters

Ketter, Sigrid¹  Sagan, Sandrine¹  Gmachl, Michael¹  Kreil, Günther¹ 

[1] Institute of Molecular Biology, Austrian Academy of Sciences, Billrothstrasse 11, A-5020 Salzburg, Austria

关键词: PH-20 protein;    Hyaluronidase;    Human sperm;    GPI;    glycosyl-phosphatidylinositol;    PI-PLC;    phosphoinositide-specific phospholipase C;   

DOI  :  10.1016/0014-5793(93)80873-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The PH-20 protein present on the membrane of guinea pig sperm was characterized using a monoclonal antibody [(1991) J. Cell Biol. 111, 2939-2949]. We have isolated the cDNA encoding the human PH-20 protein from a testis library. This cDNA was expressed in RK 13 cells using a vaccinia virus expression system. Cells expressing the human PH-20 protein possess hyaluronidase activity. Treatment with PI-PLC releases the hyaluronidase into the the medium with a concomitant large increase in enzymatic activity. These results demonstrate that the human PH-20 protein has hyaluronidase activity.

【 授权许可】

Unknown