A plasma‐membrane progesterone receptor in human sperm is switched on by increasing intracellular free calcium

FEBS Letters

Tesarik, J.¹  Mendoza, C.² 

[1] American Hospital of Paris, 63 Boulevard Victor Hugo, 92202 Neuilly sur Seine, France;Department of Biochemistry and Molecular Biology, University of Granada Faculty of Sciences, Campus Universitario Fuentenueva, 18071 Granada, Spain

关键词: Progesterone receptor;    Plasma membrane;    Nongenomic steroid effects;    Calcium mobilization;    Human sperm;    BSA-FITC;    fluorescein isothiocyanate-labeled bovine serum albumin;    [Ca2+]i;    intracellular free Ca2+;    FACS;    fluorescence-activated cell sorting;    indo 1-AM;    acetoxymethyl ester of indo 1;    progesterone-BSA-FITC;    progesterone conjugated with BSA-FITC;   

DOI  :  10.1016/0014-5793(93)80919-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Recent studies have revealed the presence of an atypical progesterone receptor on the plasma membrane of a relatively small subpopulation of human sperm cells. In this study, we show that the receptor is present in many cells in a latent form and that the receptor function in these cells is switched on asynchronously by increasing intracellular free Ca²⁺ levels. Under physiological conditions, this process occurs during sperm capacitation, but it can be mimicked by a short sperm exposure to the Ca²⁺-mobilizing agent thapsigargin. These observations suggest a role of Ca²⁺ as a modulator of nongenomic steroid effects on the cell surface.

【 授权许可】

Unknown