Progesterone action on the human sperm surface is potentiated by an egg‐associated acrosin activator

FEBS Letters

Tesarik, J.¹  Mendoza, C.²  Moos, J.³ 

[1]American Hospital of Paris, 63 Boulevard Victor Hugo, 92202 Neuilly sur Seine, France

[2]Department of Biochemistry and Molecular Biology, University of Granada Faculty of Sciences, Campus Universitario Fuentenueva, 18071 Granada, Spain

[3]Institute of Molecular Genetics, Videnska 1078, 14220 Prague, Czech Republic

关键词: Progesterone receptor;    Plasma membrane;    Nongenomic steroid effect;    Calcium influx;    Acrosin;    Acrosome reaction;    AR;    acrosome reaction;    BSA-FITC;    fluorescein isothiocyanate-labeled bovine serum albumin;    [Ca2+]i;    intracellular free Ca2+;    FACS;    fluorescence-activated cell sorting;    indo 1-AM;    acetoxymethyl ester of indo 1;    PA;    proacrosin activator;    progesterone-BSA-FITC;    progesterone conjugated with BSA-FITC;    SDS-PAGE;    sodium dodecyl sulphate-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(93)81780-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The progesterone-induced Ca²⁺ influx and acrosomal exocytosis in human sperm are recently described examples of non-genomic steroid action on the cell surface. These progesterone effects are known to be inhibited by synthetic protease inhibitors. In this study we tested a hypothesis that a physiological activator of the sperm protease acrosin modulates the sperm response to progesterone. It was found that the activator augments the amplitude of the progesterone-induced Ca²⁺ transient and accelerates the progesterone-induced acrosomal exocytosis. These observations suggest a physiological significance of the modulation of nongenomic steroid effects by protease regulators.

【 授权许可】

Unknown