Molecular characterisation, expression and localisation of human neurokinin‐3 receptor

FEBS Letters

Adami, N.¹  Arkinstall, S.J.¹  Schulz, M.F.¹  Kawashima, E.¹  Missotten, M.¹  Talabot, F.¹  Buell, G.¹  Maury, K.¹ 

[1] Glaxo Institute for Molecular Biology, 14 chemin des Aulx, Case Postale 674, 1228 Plan-les-Ouates, 1211 Geneva, 24, Switzerland

关键词: Neurokinin;    Neurokinin-3 receptor (human);    Brain localization;    Calcium mobilization;    Arachidonic acid release;   

DOI  :  10.1016/0014-5793(92)80107-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The complete amino acid sequence of the human neurokinin-3 receptor was deduced by DNA sequence analysis of human genomic fragments. Comparison of the predicted primary structure with those for the human neurokinin receptors 1 and 2 shows a highly conserved pattern of seven hydrophobic regions with maximum divergence occuring at the amino- and carboxy-termini. The position of intron-exon junctions are identical to those in other reported neurokinin genes. Using a chimeric genomic-cDNA gene, the human NK-3 receptor was expressed in Xenopus laevis oocytes where it mediates membrane conductance changes in response to its agonist, neurokinin B. More significantly, expression of the gene in mammalian cells resulted in detection of receptor binding as well as neurokinin-stimulated calcium mobilization and arachidonic acid release, all displaying the pharmacological characteristics expected of a neurokinin-3 receptor, By using the polymerase chain reaction we have shown that mRNA for the human neurokinin-3 receptor is expressed predominantly in the central nervous system.

【 授权许可】

Unknown