Two Tcp‐1‐related but highly divergent gene families exist in oat encoding proteins of assumed chaperone function

FEBS Letters

Mummert, Eckart¹  Krenz, Martina¹  Schäfer, Eberhard¹  Ehmann, Bruno¹ 

[1] Institut für Biologie II der Universität, Schänzlestr. 1, D-79104 Freiburg, Germany

关键词: cDNA sequence;    Tcp-1-related gene family;    Cytosolic 60 kDa chaperonin;    Avena sativa;   

DOI  :  10.1016/0014-5793(93)80827-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tcp-1-related sequences have been isolated from a cDNA library of etiolated 6-day-old oat (Avena sativa) seedlings. This attempt was made to obtain cDNAs of a recently published 60 kDa plant chaperone that re-folds denatured phytochrome and which was biochemically characterised as a Tcp-1-related protein [(1993) Nature 363, 644-647]. The translation of the putative coding sequence from one full-length cDNA clone displays no specific homologies to amino acid sequences known from peptide sequencing of the oat 60 kDa chaperone. Antibodies raised against the 60 kDa chaperone and over-expressed protein from one full-length coding sequence for Tcp-1 from oat show no cross-reactivity, whereas a monoclonal antibody raised against mouse Tcp-1 protein recognizes both the 60 kDa protein purified from plant extracts and over-expressed protein from Tcp-1-related cDNA sequences.

【 授权许可】

Unknown