Identification of a novel human serpin gene; cloning sequencing and expression of leupin

FEBS Letters

Worrall, D.Margaret¹  Barnes, Ruth C.¹ 

[1] Department of Biochemistry, University College Dublin, Belfield, Dublin 4, Ireland

关键词: Serpin;    Squamous cell carcinoma antigen;    Leupin;    cDNA sequence;    HeLa cell;    SCCA;    squamous cell carcinoma antigen;    PAI-2;    plasminogen activator inhibitor-2;    LEI;    leukocyte elastase inhibitor;    PTI;    placental thrombin inhibitor;    RT-PCR;    reverse transcription-polymerase chain reaction;   

DOI  :  10.1016/0014-5793(95)01015-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel serpin gene has been isolated, cloned and sequenced. A PCR amplified fragment of the gene was originally identified from human genomic DNA, and the full-length cDNA was subsequently isolated from HeLa cells and sequenced. The novel serpin is very high in protein sequence similarity (91.8%) to the squamous cell carcinoma antigen (SCCA), but contains substantial differences in the reactive site loop sequence, including a different amino acid (leucine) in the P₁ position. The gene product, named leupin, is expressed in HeLa cells, SKGIIIa cells and human placenta. The protein has a predicted M _r of 44,857 and an isoelectric point of 6.04 which is consistent with the more acidic form of SCCA associated with squamous cell carcinomas.

【 授权许可】

Unknown