Nanodetection of Head and Neck Cancer on Titanium Oxide Sensing Surface

Nanoscale Research Letters

Xiaoguang Yu¹  Yuan Seng Wu²  Jianguang Lu³  Yanan Sun³  Yan Guo³  Chao Wang³  Yu Wang³  Thangavel Lakshmipriya⁴  Subash C. B. Gopinath⁵ 

[1] Department of Biochemistry and Molecular Biology, Harbin Medical University;Department of Biochemistry, Faculty of Medicine and Biomedical Sciences, MAHSA University;Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated Hospital of Harbin Medical University;Institute of Nano Electronic Engineering, Universiti Malaysia Perlis;School of Bioprocess Engineering, Universiti Malaysia Perlis;

关键词: Squamous cell carcinoma antigen;    Interdigitated electrode;    Titanium oxide;    Gold star;    Circulating biomarker;   

DOI  :  10.1186/s11671-020-3262-x

来源: DOAJ

【 摘 要 】

Abstract Head and neck cancer is a heterogeneous disease, originating in the squamous cells lining the larynx (voice box), mouth, pharynx (throat), nasal cavity and salivary glands. Head and neck cancer diagnosis at the later stage is greatly influencing the survival rate of the patient. It makes a mandatory situation to identify this cancer at the earlier stages of development with a suitable biomarker. Squamous cell carcinoma antigen (SCC-Ag) is a circulating serum tumour biomarker, and the elevated level has been found in the head and neck cancer patients and highly correlated with the tumour volume. The present research was carried out to detect and quantify the level of SCC-Ag on titanium oxide (TiO2)-modified interdigitated electrode sensor (IDE) by SCC-Ag antibody. The detection of SCC-Ag was found at the level of 100 fM, while it was improved to 10 fM when the antibody was conjugated with gold nanostar, representing a 10-fold improvement. Interestingly, this enhancement in sensitivity is 1000-folds higher than other substrates. Moreover, the specificity analysis was carried out using two different control proteins and noticed that the antibody only recognised SCC-Ag, indicating the specific detection on IDE-TiO2 sensing surface.

【 授权许可】

Unknown