Analysis of lactase processing in rabbit

FEBS Letters

Rossi, Mauro¹  Auricchio, Salvatore¹  Russomanno, Concetta¹  Salvati, Virginia M.¹  Maiuri, Luigi¹ 

[1] Department of Pediatrics, University ‘Federico II’ of Naples, via S. Pansini 5, Naples, Italy and Istituto di Scienze dell'Alimentazione, CNR, via Roma, Avellino, Italy

关键词: Lactase;    Biosynthesis;    Proteolysis inhibition;    Suckling;    Rabbit;    LPH;    lactase-phlorizyn hydrolase (EC 3.2.1.23/62);    BFA;    brefeldin A;    MDNM;    methildeoxynojirimicin;    SDS-PAGE;    sodium dodecyl sulphate-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(93)80824-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The proteolytic processing of rabbit intestinal lactase-phlorizin-hydrolase (LPH) was studied by pulse-chase and continuous labeling experiments in organ culture from 15-day-old rabbits in the presence of glycosylation and processing inhibitors. Monensin and brefeldin A inhibited the two proteolytic cleavages of the precursor indicating that they are post-Golgi events as previously reported for the unique cleavage of LPH in man [1]. The inhibition was not related to a concomitant alteration glycosylation; in fact, if trimming was blocked by MDNM the abnormal glycosylated precursor was proteolytically processed normally. Finally the use of the anti-microtubular drug colchicine strongly inhibited both cleavages and caused accumulation of the complex-glycosylated precursor form in the brush border fraction indicating that proteolytic events depend on intact microtubule (transport).

【 授权许可】

Unknown