In vitro biosynthesis of lactase in suckling and adult rabbits

FEBS Letters

Rossi, Mauro¹  Auricchio, Salvatore¹  Russomanno, Concetta¹  Salvati, Virginia M.¹  Capparelli, Rosanna²  Maiuri, Luigi¹ 

[1] Department of Pediatrics, University ‘Federico II’ of Naples, via S. Pansini 5, Naples, Italy;Istituto di Produzione Animale, Facoltà di Agraria, Università 'Federico II' di Napoli, via Universita 100, Portici, Naples, Italy

关键词: Lactase;    Biosynthesis;    Enzyme inactivation;    Suckling and adult rabbit;    LPH;    lactase-phlorizin hydrolase (EC 3.2.1.23/62);    SDS-PAGE;    sodium dodecyi sulphate-polyacrylamide gel electrophoresis;    TCA;    trichloroacetic acid;    HPLC;    high performance liquid chromatography;   

DOI  :  10.1016/0014-5793(93)80203-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Steady state forms, levels and the in vitro biosynthesis of lactase-phlorizin hydrolase (LPH) proteins have been studied in proximal and middle intestine of suckling and adult rabbits. In most adult tissues the lactase activity and the LPH protein content were low and the synthesis rate of the 200 kDa lactase precursor was reduced in comparison to suckling tissues. In a few tissues with low enzymatic activity the LPH protein content was relatively high, and high lactase synthesis occurred. In addition, the ratio (labeled lactase)/(lactase protein) was lower in the middle jejunum of the adult rabbit than in the proximal region. Both decreased synthesis of LPH precursor and increased turnover or inactivation of the enzyme may cause the decline of the lactase activity.

【 授权许可】

Unknown