Isolation and Molecular Identification of New Kluyveromyces lactis Strains Producing High Levels of Lactase and Invertase Enzymes

Journal of Applied Biological Sciences

Sezai TÜRKEL¹  Nükhet KAYAKENT¹ 

关键词: Kluyveromyces lactis;    Lactase;    Invertase;    Yeast identification;    PCR-RFLP;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

SixdifferentnewKluyveromyceslactisstrainswereisolatedfromdifferentdairyproductsfromBursaprovince,Turkey.LactaseandinvertaseenzymeactivitiesofnewK.lactisstrainsweredeterminedunderdifferentgrowthconditions.OurresultsindicatethatbothlactaseandinvertaseenzymeactivitiesofnewK.lactisstrainsaremuchhigherthanthestandardK.lactis(ATCC8585).LactaseandinvertaseenzymesareexpressedathighlevelsunderglucoserepressedgrowthconditionsinsomeoftheseK.lactisstrains.InvertaseactivitiesofnewK.lactisstrainsare10-foldhigherthanthestandardK.lactis(ATCC8585)strain.Additionally,itwasshownthatthetwoofthenewK.lactisstrainshave25%shorterduplicationtimesthanthenormalK.lactis(ATCC8585).OurresultsindicatethatnewK.lactisstrainsisolatedfromthelocaldairyproductsoftheBursaregionofTurkeyhavedistinctmetabolicfeatureswithrespecttotheregulationofGAL/LACregulonofK.lactis.

【 授权许可】

Unknown