Different factors bind to the regulatory region of the Na+,K+‐ATPase α1‐subunit gene during the cell cycle

FEBS Letters

Watanabe, Yuko²  Kawakami, Kiyoshi²  Yanagisawa, Ken¹  Tominaga, Shin-ichi¹  Nagano, Kei² 

[1] Departments of Biochemistry II, Jichi Medical School, Yakushiji, Minamikawachi, Kawachi, Tochigi 329-04, Japan;Departments of Biology, Jichi Medical School, Yakushiji, Minamikawachi, Kawachi, Tochigi 329-04, Japan

关键词: Na+;    K+-ATPase;    Cell cycle;    Transcription factor;    Gel retardation assay;    Nuclear extract;    Serum stimulation;    ARE;    ATP1A1 regulatory element;    DTT;    dithiothreitol;    EDTA;    ethylenediaminetetraacetic acid;    EGTA;    ethylene glycolbis(β-aminoethyl ether) N;    N;    N';    N'-tetraacetic acid;    Na+;    K+-ATPase;    sodium- and potassium-dependent adenosine triphosphatase;    PMSF;    phenylmethylsulfonyl fluoride;   

DOI  :  10.1016/0014-5793(93)80740-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Three factors that bind to the positive regulatory region (ARE) of the Na⁺,K⁺-ATPase α1-subunit gene were shown to be present in growing BALB/c-3T3 cells as shown by the gel retardation assay pattern in which three specific complexes (C1, C2 and C3) were identified. The complexes are similar to those observed in MDCK cell nuclear extracts in which linker substitution mutations in the competitor gave parallel specific effects in both cells. During the process of the cell growth cycle, the relative mobility of C3 was altered, and the amount of C1 decreased in the G₀ state. All three complexes (C1, C2 and C3) disappeared and other specific complexes with higher mobilities were alternatively observed at 6h after serum stimulation and thereafter. The expression of the mRNA for the α1-subunit gene was repressed at G₀ and gradually increased after serum stimulation. These results suggest that different sets of factors are responsible for the transcription of the gene at different stages of the cell cycle.

【 授权许可】

Unknown