Selectivity of phospholipase C isozymes in growth factor signaling

FEBS Letters

Chae, Soo-Wan³  Kim, Uh-Hyun¹  Cho, Yee-Sook¹  Han, Myung-Kwan¹  Park, Chung-Ung² 

[1] Departments of Biochemistry, Chonbuk National University Medical School, Chonju 560-182, Korea;Department of Molecular Biology, College of Natural Sciences, Chonbuk National University, Chonju 560-182, Korea;Departments of Pharmacology, Chonbuk National University Medical School, Chonju 560-182, Korea

关键词: Growth factor;    Phospholipase C isozyme;    Thrombin receptor;    Ca2+ mobilization;    Xenopus oocyte;   

DOI  :  10.1016/0014-5793(93)80689-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Xenopus laevis oocytes were injected with mRNA extracted from growth factor-responsive CCL39, Chinese hamster lung fibroblasts. The expression of functional growth factor receptors on the oocytes was demonstrated by growth factor-induced ⁴⁵Ca²⁺ efflux. To determine the isozyme(s) of phospholipase C (PLC) coupled to growth factor receptors, growth factor-induced ⁴⁵Ca²⁺ efflux were measured following coinjection of mRNA from CCL39 cells with PLC antibodies. PLC-γ1 antibody did not lead to loss of ⁴⁵Ca²⁺ efflux induced by thrombin but resulted in loss of that induced by platelet-derived growth factor (PDGF). In contrast, PLC-δ1 antibody did not block PDGF-induced ⁴⁵Ca²⁺ efflux but led to inhibition of thrombin-induced ⁴⁵Ca²⁺ efflux. PLC-β1 antibody did not affect Ca²⁺ efflux by the treatment of either thrombin or PDGF. These results suggest that these growth factor receptors are coupled to specific effectors, i.e. thrombin receptor to PLC-δ1 and PDGF receptor to PLC-γ1.

【 授权许可】

Unknown