Long‐term effects of thrombin require sustained activation of the functional thrombin receptor

FEBS Letters

Sraer, Jean Daniel¹  Nguyen, Geneviève¹  Rondeau, Eric¹  He, Ci-Jiang¹  Zacharias, Ute¹ 

[1] INSERM U64, Hôpital Tenon, 4 rue de la Chine, 75020 Paris, France

关键词: Thrombin;    Thrombin receptor;    TRAP;    Aminopeptidase;    Glomerular epithelial cell;    HGEC;    human glomerular epithelial cells;    TRAP;    thrombin receptor agonist peptide;    u-PA;    urokinase-type plasminogen activator;    t-PA;    tissue-type plasminogen activator;    PAI-1;    plasminogen activator inhibitor type 1;    EGF;    epidermal growth factor;    bFGF;    basic fibroblast growth factor;   

DOI  :  10.1016/0014-5793(93)81716-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Thrombin is a potent activator of human glomerular epithelial cells (HGEC). Here we compare short-term and long-term effects of thrombin and thrombin receptor agonist peptide (TRAP) which selectively activates the functional thrombin receptor. TRAP, as thrombin, increases intracellular free Ca²⁺ concentration and acts synergistically with growth factors possessing tyrosine kinase receptors on DNA synthesis. Thrombin induces synthesis of proteins of the fibrinolytic system and cell proliferation if it is present for at least 8 h. TRAP alone does not stimulate protein synthesis and is not mitogenic. However, in the presence of the aminopeptidase inhibitor amastatin all long-term effects of thrombin can be fully mimicked by TRAP. In conclusion, different effects of thrombin and TRAP may be related to the degradation of TRAP by cellular ectoenzymes. The recently cloned thrombin receptor accounts for early intracellular signals and long-term cellular effects that require sustained activation of this receptor.

【 授权许可】

Unknown