A G‐protein‐coupled 130 kDa phospholipase C isozyme, PLC‐β4, from the particulate fraction of bovine cerebellum

FEBS Letters

Kim, Yong¹  Min, Do Sik¹  Ryu, Sung Ho¹  Suh, Pann-Ghill¹  Lee, Young Han¹ 

[1] Department of Life Science, Pohang University of Science and Technology, P.O. Box 125, Pohang, 790-600, South-Korea

关键词: Phospholipase C isozyme;    Bovine cerebellum;    G-protein-dependent activation;   

DOI  :  10.1016/0014-5793(93)80293-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A 130 kDa PLC isozyme was purified from the particulate fraction of bovine cerebellum. This PLC was recognized by a polyclonal antiserum generated against the purified 97 kDa PLC-β4. Reconstitution of the purified 130 kDa PLC with the membranes of C₆ Bu-1 cells in the presence of GTPγS or Alp₄ ⁻ resulted in PLC activation as well as the association of PLC with the membranes. Both the association and activation were revoked when the membrane was washed with 2 M KC1. The 97 kDa PLC-β4 did not associate with membranes. These data suggest that the 130 kDa PLC is the intact form of PLC-β4 the activity of which is likely to be regulated by a G-protein on the membrane.

【 授权许可】

Unknown