Cloning, sequence and expression of the gene encoding the malolactic enzyme from Lactococcus lactis

FEBS Letters

Dequin, Sylvie¹  Barre, Pierre¹  Blondin, Bruno¹  Ansanay, Virginie¹ 

[1] Laboratoire de Microbiologie et Technologie des Fermentations, Institut des Produits de la Vigne, INRA, 2 Place Viala, F-34060 Montpellier Cedex 1, France

关键词: Gene cloning;    Malolactic enzyme;    Malic enzyme;    Lactococcus lactis;    Saccharomyces cerevisiae;   

DOI  :  10.1016/0014-5793(93)80488-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Many lactic acid bacteria can carry out malolactic fermentation. This secondary fermentation is mediated by the NAD- and Mn²⁺-dependent malolactic enzyme, which catalyses the decarboxylation of l-malate to l-lactate. The gene we call mleS, coding for malolactic enzyme, was isolated from Lactococcus lactis. The mleS gene consists of one open reading frame capable of coding for a protein with a calculated molecular mass of 59 kDa. The amino acid sequence of the predicted MleS gene product is homologous to the sequences of different malic enzymes. Bacterial and yeast cells expressing the malolactic gene convert l-malate to l-lactate.

【 授权许可】

Unknown