Studies on cytotoxic and clot lysis activity of probiotically fermented cocktail juice prepared using Camellia sinensis and Punica grantum

14th International Conference on Science, Engineering and Technology

自然科学;工业技术

Biswas, Ananya^1 ; Deori, Meenakshi^1 ; Nivetha, A.^1 ; Mohansrinivasan, V.^1

School of Bioscience and Technology, VIT University, Vellore, TamilNadu

632014, India^1

关键词: Analytical studies;    Camellia sinensis;    Colon cancer cells;    Fermented juices;    Free radical scavenging activity;    Lactobacillus plantarum;    Lactococcus lactis;    Organic acid productions;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/263/2/022044/pdf
DOI  :  10.1088/1757-899X/263/2/022044

来源: IOP

【 摘 要 】

In the current research the effect of probiotic microorganisms viz; Lactococcus lactis and Lactobacillus plantarum on fermentation of Camellia sinensis and Punica grantum was studied. In vitro test were done to analyze the anticancer, antioxidant and atherosclerosis (clot lysis) properties of fermented juice. The juice was fermented for 48 and 96h, during which concentration of phenolic content, total acid content and free radical scavenging activity of the sample was analyzed by DPPH assay (α, α-diphenyl-β-picrylhydrazyl). Dropping of pH was observed after 48 h of fermentation. The clot lysis activity was found to be 80 % in 100μl concentration of fermented cocktail juice. The 96 h fermented sample has shown around 70% inhibition against colon cancer cell lines. Analytical study of HPLC proves the organic acid production such as ascorbic acid in superior amount for 96h of fermented sample, Based on the retention time, the corresponding peaks were detected at 4.919 and 4.831 min.