Metabolic analysis of S. cerevisiae strains engineered for malolactic fermentation

FEBS Letters

Dequin, S²  Barre, P²  Camarasa, C²  Ansanay, V²  Dulau, L¹  Bony, M¹  Bidart, F¹ 

[1] Lallemand S.A. Complexe scientifique de Rangueil, Hall Biotechnologie III, BP 4412 F-31405 Toulouse cedex, France;IPV-Laboratoire de Microbiologie et Technologie des Fermentations, Inra, 2 Place Viala F-34060 Montpellier cedex 01, France

关键词: Malate metabolism;    (Saccharomyces cerevisiae);    Malate transport;    Malolactic enzyme;    Malolactic fermentation;    Enology;   

DOI  :  10.1016/S0014-5793(97)00637-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A complete malolactic fermentation was achieved using Saccharomyces cerevisiae strains coexpressing the genes mleS and mae1 coding for the Lactococcus lactis malolactic enzyme and the Schizosaccharomyces pombe malate permease under the control of yeast promoters. The expression level of mae1 greatly influences the kinetics of the reaction by controlling the rate of malate uptake meanwhile a high expression level of mleS induces a partial consumption of malate derived from glucose by the malolactic enzyme. A strain expressing several copies of mae1 and one copy of mleS degrades 3 g/l of malate almost exclusively through the malolactic pathway in 4 days under enological conditions, without metabolic side effects.

【 授权许可】

Unknown