FEBS Letters | |
Superoxide enhances the spread of HIV‐1 infection by cell‐to‐cell transmission | |
Kimura, Takuro1  Ikuta, Kazuyoshi1  Kameoka, Masanori1  | |
[1] Section of Serology, Institute of Immunological Science, Hokkaido University, Kita-ku, Sapporo 060, Japan | |
关键词: HIV-1; Superoxide; Syncytium formation; Antioxidant; AIDS; HIV-1; human immunodeficiency virus type 1; O2 −; Superoxide; AIDS; acquired immune deficiency syndrome; IF; immunofluorescence; RT; reverse transcriptase; PMA; phorbol 12-myristate 13-acetate; SOD; Superoxide dismutase; MOI; multiplicity of infection; ceruloplasmin; | |
DOI : 10.1016/0014-5793(93)80322-L | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Oxidative stress is thought to be involved in the progression of human immunodeficiency virus type 1 (HIV-1)-induced disease. We examined the effect of Superoxide (O2 −) on HIV-1 spread in cultured human CD4+ cell lines. The O2 − significantly enhanced cell-to-cell transmission of HIV-1, although its effect on HIV-1 replication was not evident, presumably due to its cytostatic activity. The effect was notable on the HIV-1 transmission from macrophages to T lymphocytes by endogenous, macrophage-generated O2 −. This amplification was specifically reduced to the steady-state level by antioxidants, and further to the basal level by anti-CD4 antibodies, indicating the specificity of O2 − for enhancing HIV-1 spread by cell-to-cell transmission.
【 授权许可】
Unknown
【 预 览 】
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