Superoxide enhances the spread of HIV‐1 infection by cell‐to‐cell transmission

FEBS Letters

Kimura, Takuro¹  Ikuta, Kazuyoshi¹  Kameoka, Masanori¹ 

[1] Section of Serology, Institute of Immunological Science, Hokkaido University, Kita-ku, Sapporo 060, Japan

关键词: HIV-1;    Superoxide;    Syncytium formation;    Antioxidant;    AIDS;    HIV-1;    human immunodeficiency virus type 1;    O2 −;    Superoxide;    AIDS;    acquired immune deficiency syndrome;    IF;    immunofluorescence;    RT;    reverse transcriptase;    PMA;    phorbol 12-myristate 13-acetate;    SOD;    Superoxide dismutase;    MOI;    multiplicity of infection;    ceruloplasmin;   

DOI  :  10.1016/0014-5793(93)80322-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Oxidative stress is thought to be involved in the progression of human immunodeficiency virus type 1 (HIV-1)-induced disease. We examined the effect of Superoxide (O₂ ⁻) on HIV-1 spread in cultured human CD4⁺ cell lines. The O₂ ⁻ significantly enhanced cell-to-cell transmission of HIV-1, although its effect on HIV-1 replication was not evident, presumably due to its cytostatic activity. The effect was notable on the HIV-1 transmission from macrophages to T lymphocytes by endogenous, macrophage-generated O₂ ⁻. This amplification was specifically reduced to the steady-state level by antioxidants, and further to the basal level by anti-CD4 antibodies, indicating the specificity of O₂ ⁻ for enhancing HIV-1 spread by cell-to-cell transmission.

【 授权许可】

Unknown