FEBS Letters | |
Inhibitory effect of neopterin on NADPH‐dependent superoxide‐generating oxidase of rat peritoneal macrophages | |
Kojima, Shuji1  Icho, Takeshi1  Kitabatake, Katsuaki2  Nomura, Takafumi1  Kubota, Kazuhiko1  Kajiwara, Yoshio2  | |
[1] Research Institute for Biosciences, Science University of Tokyo, 2669 Yamazaki, Nodashi, Chiba 278, Japan;Applied Technology Research Laboratory, Asahi Breweries, Ltd., 2-13-1 Ohmorikita, Ohtaku, Tokyo 143, Japan | |
关键词: Neopterin; Antioxidant; Superoxide; NADPH-oxidase; NP; neopterin; NPH4; 5; 6; 7; 8-tetrahydroneopterin; NPH2; 7; 8-dihydroneopterin; NPH2TP; 7; 8-dihydroneopterin triposphate; GTP; guanosine triphosphate; BP; biopterin; BPH4; 5; 6; 7; 8-tetrahydrobiopterin; IFN-γ; interferon-γ; PMA; phorbol myristate acetate; X/XOD; xanthine/xanthine oxidase; PK.C; protein kinase C; SDS; sodium dodecyl sulfate; SOD; Superoxide dismutase; FAD; flavin adenine dinucleiotide; | |
DOI : 10.1016/0014-5793(93)80207-B | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The effect of the oxidized form of neopterin (NP) on the NADPH-dependent superoxide-generating oxidase (NADPH-oxidase) was investigated in both whole-cell and cell-free activation systems by using peritoneal macrophages of rats which had received an intraperitoneal injection of mineral oil. In the whole-cell system, NP remarkably inhibited the generation of Superoxides in macrophages stimulated with phorbol myristate acetate (PMA). NP also showed an significant suppression of the activation of superoxide-generating NADPH-oxidase in the cell-free system using solubilized membranes and sodium dodecyl sulfate (SDS) as a stimulant. The 50%-inhibitory concentration (IC50) of NP was about 1 μM in both assay systems. In a kinetic study, competitive inhibition of the NADPH-oxidase by NP was observed in the cell-free system with a calculated inhibition constant (K i) of 6.50 μM. These findings suggest that NP may play an important role in the suppression of Superoxide generation via the inhibition of the NADPH-oxidase in phagocytes.
【 授权许可】
Unknown
【 预 览 】
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