A complex of rab3A, SNAP‐25, VAMP/synaptobrevin‐2 and syntaxins in brain presynaptic terminals

FEBS Letters

Odani, Shoji²  Saisu, Hideo³  Abe, Teruo³  Tsugita, Akira¹  Horikawa, Hiroshi P.M.³  Sekine, Yoko³  Ishizuka, Toru³ 

[1] Institute of Biosciences, Science University of Tokyo, 287 Noda, Japan;Department of Biology, Faculty of Science, Niigata University, Niigata 951, Japan;Department of Neurochemistry, Brain Research Institute, Niigata University, Asahimachi 1, Niigata 951, Japan

关键词: Rab3A/smg25A;    Syntaxin;    VAMP/synaptobrevin-2;    SNAP-25;    Neurotransmitter release;   

DOI  :  10.1016/0014-5793(93)80281-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two monoclonal antibodies (SPM-1 and SPM-2) immunoprecipitate brain N-type calcium channels. On immunoaffinity chromatography of digitonin extracts of bovine brain membranes on SPM-1- and SPM-2-Sepharose, proteins of 36 (syntaxins A and B), 28 and 19 kDa are specifically retained by both columns. Here we show that the 19 and 28 kDa bands contain VAMP/synaptobrevin-2, and rab3A/smg25A and SNAP-25, respectively. Since SPM-1 and SPM-2 recognize only syntaxins and the 28 kDa band (rab3A/smg25A and SNAP-25), respectively, the results indicate that all these proteins form a complex. Our results suggest tight linkage between the components involved in neurotransmitter release.

【 授权许可】

Unknown