SNAP‐25 is present in a SNARE complex in adrenal chromaffin cells

FEBS Letters

Burgoyne, Robert D.¹  Roth, Dagmar¹ 

[1] The Physiological Laboratory, University of Liverpool, PO Box 147, Liverpool L69 3BX, UK

关键词: Exocytosis;    Syntaxin;    N-Ethylmaleimide-sensitive fusion protein (NSF);    SNAP;    Synaptotagmin;    Secretion;    BoNT;    botulinum neurotoxin;    NSF;    N-ethylmaleimide-sensitive fusion protein;    PNP;    post-nuclear pellet;    PNS;    post-nuclear supernatant;    SNAP-25;    synaptosomal-associated protein 25 kDa;    SNAP;    soluble NSF attachment protein;    SNARE;    SNAP receptor;   

DOI  :  10.1016/0014-5793(94)00833-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

SNAP-25 (synaptosomal-associated protein 25 kDa) is a target for botulinum neurotoxins A and E, which both inhibit neurotransmitter release, and was recently identified together with syntaxin and synaptobrevin as receptors for NSF and α-SNAP. We show that SNAP-25 was enriched in the microsomal fraction from adrenal medulla, although the level of SNAP-25 in adrenal medullary microsomes was about 20-fold less than in brain microsomes. Immunocytochemistry confirmed the presence of SNAP-25 in cultured chromaffin cells and showed plasma membrane staining. Using immunoprecipitation, we found that SNAP-25 was present in a complex with syntaxin, synaptobrevin, synaptotagmin, NSF, α-SNAP and other unidentified polypeptides. These data indicate that SNAP-25 in chromaffin cells is present in a complex similar to that identified in brain.

【 授权许可】

Unknown