A novel ryanodine sensitive calcium release mechanism in cultured human myometrial smooth‐muscle cells

FEBS Letters

Lynn, Stephen¹  Morgan, Joanna M.¹  Greenwell, John R.¹  Gillespie, James I.¹ 

[1] Department of Physiological Sciences, The Medical School, The University, Newcastle upon Tyne, NE2 4HH, UK

关键词: Ryanodine;    Caffeine;    Intracellular calcium;    Myometrial smooth muscle;   

DOI  :  10.1016/0014-5793(93)80279-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In cultured human myometrial cells application of caffeine (1–30 mM) did not result in an elevation of intracellular Ca²⁺ ([Ca²⁺]_i). Caffeine was found to reversibly inhibit both spontaneous and agonist-induced repetitive rises in [Ca²⁺]_i possibly as a consequence of its ability to interfere with the binding of inositol trisphosphate (IP₃) to the receptor on the sarcoplasmic reticulum. Brief applications of ryanodine (1–10μM) were observed to elevate [Ca²⁺]_i and repeated exposures to ryanodine could elicit Ca²⁺ transients of similar magnitude. Ryanodine was also observed to mobilise Ca²⁺ in cells bathed in nominally Ca²⁺-free solution. These observations suggest the presence of a novel type of ryanodine-sensitive Ca²⁺-induced Ca²⁺ release (R-CICR) system in human myometrial cells.

【 授权许可】

Unknown