FEBS Letters | |
Activation and inhibition of the calcium gate of sarcoplasmic reticulum by high‐affinity ryanodine binding | |
Migala, Andrea1  Hasselbach, Wilhelm1  | |
[1] Max Planck Institute for Medical Research, Department of Physiology, Heidelberg, FRG | |
关键词: Sarcoplasmic reticulum; Ryanodine; Caffeine; Ca2 + uptake; Ca2 + release; | |
DOI : 10.1016/0014-5793(87)80364-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The occupancy of high-affinity ryanodine-binding sites of isolated heavy sarcoplasmic reticulum vesicles occurring in concentrated salt solutions affects ATP-dependent calcium accumulation and caffeine-induced calcium release. The initial suppression of calcium uptake is followed by a marked uptake activation resulting in a reduction of the final calcium level in the medium. Simultaneously, caffeine-induced calcium release is blocked. The dependence of inhibition of calcium uptake and caffeine-induced calcium release observed in assay media containing physiological concentrations of magnesium and ATP on the concentration of ryanodine corresponds to the drug's effectiveness in living muscles.
【 授权许可】
Unknown
【 预 览 】
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