Activation and inhibition of the calcium gate of sarcoplasmic reticulum by high‐affinity ryanodine binding

FEBS Letters

Migala, Andrea¹  Hasselbach, Wilhelm¹ 

[1] Max Planck Institute for Medical Research, Department of Physiology, Heidelberg, FRG

关键词: Sarcoplasmic reticulum;    Ryanodine;    Caffeine;    Ca2 + uptake;    Ca2 + release;   

DOI  :  10.1016/0014-5793(87)80364-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The occupancy of high-affinity ryanodine-binding sites of isolated heavy sarcoplasmic reticulum vesicles occurring in concentrated salt solutions affects ATP-dependent calcium accumulation and caffeine-induced calcium release. The initial suppression of calcium uptake is followed by a marked uptake activation resulting in a reduction of the final calcium level in the medium. Simultaneously, caffeine-induced calcium release is blocked. The dependence of inhibition of calcium uptake and caffeine-induced calcium release observed in assay media containing physiological concentrations of magnesium and ATP on the concentration of ryanodine corresponds to the drug's effectiveness in living muscles.

【 授权许可】

Unknown