Mechanisms of the inhibition of reverse transcription by unmodified and modified antisense oligonucleotides

FEBS Letters

Yamamoto, Naoki¹  Takaku, Hiroshi³  Yokoyama, Shigeyuki²  Kim, Sang-Gug³  Nakashima, Hideki¹  Sakamoto, Kensaku²  Hatta, Toshifumi³ 

[1] Department of Microbiology, Tokyo Medical and Dental University School of Medicine, Yushima, Bunkyo-ku, Tokyo 113, Japan;Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113, Japan;Department of Industrial Chemistry, Chiba Institute of Technology, Tsudanuma, Narashino, Chiba 275, Japan

关键词: Reverse transcriptase;    Antisense oligonucleotide;    Rabbit β-globin;    Retrovirus;    Polyacrylamide gel;   

DOI  :  10.1016/0014-5793(93)80264-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We demonstrated that unmodified and modified (phosphorothioate) oligonucleotides prevent cDNA synthesis by AMV or HIV reverse transcriptases. Antisense oligonucleotide/RNA hybrids specifically arrest primer extension. The blockage involves the degradation of the RNA fragment bound to the antisense oligonucleotide by the reverse transcriptase-associated RNase H activity. However, the phosphorothioate oligomer inhibited polymerization by binding to the AMV RT rather than to the template RNA, whereas there was no competitive binding of the phosphorothioate oligomer on the HIV RT during reverse transcription.

【 授权许可】

Unknown