Non‐antisense cellular responses to oligonucleotides

FEBS Letters

Mayat, Ebrahim¹  Payrastre, Bernard²  Massoulié, Jean¹  Anselmet, Alain¹  Layer, Paul G³  Wietek, Stefan¹ 

[1] CNRS UMR 8544, Laboratoire de Neurobiologie, Ecole Normale Supérieure, 46 rue d'Ulm, 75005 Paris, France;Unité INSERM 326, CHU Purpan, 31000 Toulouse, France;Darmstadt University of Technology, Institute of Zoology, Schnittspahnstrasse 3, D-64287 Darmstadt, Germany

关键词: Antisense oligonucleotide;    Double strand;    Cell aggregation;    G-quartet;    Neurite;    Platelet;    AChE;    acetylcholinesterase;    BChE;    butyrylcholinesterase;    D.I.C.;    days in culture;    DMEM;    Dulbecco's modified Eagle's medium;    DOTMA;    N-[1-(2;    3 dioleyloxy)propyl]-N;    N;    N-trimethylammonium chloride;   

DOI  :  10.1016/S0014-5793(01)03248-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Oligonucleotides induce various cellular responses which are not due to the blockade of protein synthesis by an antisense mechanism. Oligonucleotides presenting double-stranded or G-quartet structures (ribo- or deoxyribonucleotides, phosphodiester or phosphorothioated) induce retraction of neurites and aggregation of chicken retinal cells within 10–20 h. This effect is reversible, non-toxic; it appears to require internalization and can be mimicked by treatment of the cells with an RGDS peptide. The oligonucleotides appear to trigger a cascade of intracellular events, affecting the adhesive properties of integrins. In addition, a subset of oligonucleotides induced platelet aggregation, probably through their interaction with membrane receptors. Recognition of these effects is important for the design and interpretation of antisense experiments.

【 授权许可】

Unknown