A phosphorothioate oligonucleotide blocks reverse transcription via an antisense mechanism

FEBS Letters

Moreau, Serge¹  Toulmé, Jean-Jacques¹  Boiziau, Claudine¹ 

[1] Laboratoire de Biophysique Moléculaire, INSERM U 386, Université de Bordeaux II, 146 rue Léo Saignat, 33 076 Bordeaux cédex, France

关键词: Antisense oligonucleotide;    Inhibition mechanism;    Retrovirus;    RNase H;    RT;    reverse transcriptase;    R Nase H;    ribonuclease H;    AMV;    avian myeloblastosis virus;    MMLV;    Moloney murine leukemia virus;    MMLV H− RT;    MMLV RT devoid of R Nase H activity;    HIV;    human immunodeficiency virus;    PS;    phosphorothioate;    PO;    phosphodiester;    nt;    nucleotide;   

DOI  :  10.1016/0014-5793(94)80145-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have studied the inhibition by a phosphorothioate oligodeoxynucleotide (17PScap) of cDNA synthesis performed by either avian or murine reverse transcriptase. Three different mechanisms of inhibition were identified: at low concentrations (⊃100 nM), the cleavage of the RNA template by the retroviral RNase H at the level of the RNA/17PScap duplex accounted for most of the effect, whereas hybrid-arrested cDNA synthesis by an RNase H-independent mechanism marginally contributed to the inhibition. Both mechanisms were sequence-specific. Above 100 nM, the overall cDNA synthesis was reduced in a non-specific manner.

【 授权许可】

Unknown