Ribosomal proteins, TL4 and TL5, from Thermus thermophilus form hybrid complexes with 5 S ribosomal RNA from different microorganisms

FEBS Letters

Gongadze, G.M.¹  Garber, M.B.¹  Tishchenko, S.V.¹  Sedelnikova, S.E.¹ 

[1] Department of Structure and Function of Ribosomes, Institute of Protein Research, Russian Academy of Sciences, 142292 Pushchino, Moscow Region, Russian Federation

关键词: 5 S RNA;    Hybrid complex;    Thermus thermophilus;    Ribosomal protein;   

DOI  :  10.1016/0014-5793(93)80916-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hybrid complexes of the ribosomal proteins, TL4 and TL5, from Thermus thermophilus with 5 S ribosomal RNA from Escherichia coli and Bacillus stearothermophilus have been prepared. There was no competition between the two proteins for the binding sites. Stoichiometry of 5 S RNA binding for both proteins was 1:1 (protein/RNA). The TL4 protein competed with the E. coli ribosomal L5 protein, and the TL5 protein competed with theE. coli ribosomal proteins, L18 and L25, for binding with 5 S RNA.

【 授权许可】

Unknown