Ribosomal protein L22 from Thermus thermophilus: sequencing overexpression and crystallisation

FEBS Letters

Al-Karadaghi, Salam¹  Garber, Maria B.²  Vysotskaya, Valentina S.²  Davydova, Natalia L.¹  Gryaznova, Olga I.¹  Liljas, Anders¹  Jonsson, Bengt-Harald³  Mashchenko, Oksana V.² 

[1] Molecular Biophysics, University of Land, Chemicuf Center POB 124, S-221 00 Lund, Sweden;Department of Structure and Function of the Rihosome, Institute of Protein Research, Russian Academy of Sciences, 142292 Pushchino, Moscow Region, Russian Federation;Department of Biochernistrv, Urmeå Unirersity, S-90187 Umeå, Sweden

关键词: Ribosomal protein;    Overexpression;    Purification;    Crystallisation;    Thermus thermophilus;   

DOI  :  10.1016/0014-5793(95)00755-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The gene for the ribosomal protein L22 from Thermus thermophilus has been sequenced and overexpressed in Escherichia coli. A multiple sequence alignment was carried out for all proteins of the L22 family reported so far. The recombinant protein was purified and crystallized. The crystals belong to the space group P2₁2₁2₁ with cell parameters of .

【 授权许可】

Unknown