Cloning, overexpression, purification and crystallisation of ribosomal protein L9 from Bacillus stearothermophilus

FEBS Letters

Dauter, Zbigniew¹  Vorgias, Constantin E.¹  Wilson, Keith S.¹  Kingswell, Adrian J.¹ 

[1] European Molecular Biology Laboratory (EMBL), c/o DESY, Notkestrasse 85, 2000 Hamburg 52, Germany

关键词: Bacillus stearothermophilus;    Ribosomal protein;    Gene sequence;    Crystallisation;   

DOI  :  10.1016/0014-5793(91)80974-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cloning, sequencing and overexpression of the gene coding for Bacillus stearothermophilus ribosomal protein L9 is described. The sequence corresponds directly to that presented for the protein itself by classical methods, differing at only a few amino acid positions. The purification and crystallisation of the corresponding L9 protein is presented. The crystals are isomorphous to those described for L9 obtained by conventional methods.

【 授权许可】

Unknown